The Communications, Energy and Paperworkers Union said Thursday another 80 employees will also be affected by the consolidation and movement of work and may have to move to keep their jobs.
The union said the changes affect workers primarily in Ottawa, Toronto and London, Ont.
Bell said several call centres that support business and wholesale customers will be centralized into fewer major locations in Quebec and Ontario, which will help the centres operate more efficiently.
"These changes that will take place over the next two months and affected employees will have the opportunity to seek other positions within Bell," said spokesman Jason Laszlo.
"Keep in mind that Bell employs more than 50,000 people across Canada, and our service teams are growing."
Bell Canada's parent company is BCE Inc. (TSX:BCE).